An analysis of chromosomal DNA using the Southern hybridization technique does not use:
Correct answer: D. PCR
- A. Electrophores
- B. Blotting
- C. Autoradiography
- D. PCR
Explanation
Southern blotting is based on the principle of separation of DNA fragments by gel electrophoresis followed by the identification of labelled probe hybridization. The DNA fragments are separated based on their size and charge during electrophoresis.
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