Moderate

Bacillus thuringiensis forms protein crystals that contain insecticidal protein. This protein:

Correct answer: A. Binds with epithelial cells in the midgut of the insect pest ultimately killing it

  • A. Binds with epithelial cells in the midgut of the insect pest ultimately killing it
  • B. Is coded by several genes including the gene cry
  • C. Is activated by acid pH of the foregut of the insect pest
  • D. Does not kill the carrier bacterium which is itself resistant to this toxin

Explanation

Soil bacterium Bacillus thuringiensis produces proteins that kill certain insects like lepidopterans (tobacco budworm, armyworm), coleopterans (beetles), and dipterans (flies, mosquitoes). B. thuringiensis forms some protein crystals which contain a toxic insecticidal protein. This toxin does not kill the Bacillus (bacterium) because it exists as inactive protoxins in them. But, once an insect ingests it, it is converted into an active form of toxin due to the alkaline pH of the alimentary canal. The activated toxin binds to the surface of midgut epithelial cells and creates pores that cause swelling and lysis and finally cause the death of the insect.

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