Moderate

Genes of interest can be selected from a genomic library by using:

Correct answer: B. DNA probes

  • A. Cloning vectors
  • B. DNA probes
  • C. Gene targets
  • D. Restriction enzymes

Explanation

A gene bank or genomic library is a complete collection of cloned DNA fragments that comprises the entire genome of an organism. Molecular probes are small DNA segments that are used to detect the presence of complementary sequences in nucleic acid samples in the genomic library. These are usually formed of 200­500 nucleotide sequences. These segments or probes are labeled either with radioactive or nonradioactive compounds. Probes with DNA sequences complementary to the gene to be isolated are used. They bind with the desired gene, making it visible and help in isolating it from the library.

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Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.

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