If one of the following components is missing , bacteria can not increase the number of its plasmid copies.

Explanation

An essential feature of bacterial plasmids is their ability to replicate as autonomous genetic elements in a controlled way within the host. Therefore, they can be used to explore the mechanisms involved in DNA replication and to analyze the different strategies that couple DNA replication to other critical events in the cell cycle therefore they can replicate to increase the number of plasmid.