If the primer annealing temperature is increased to 94°C . What will happen ?
Correct answer: C. No Annealing
- A. Annealing
- B. Extension
- C. No Annealing
- D. Primer-dimer formation
Explanation
Primer annealing is a critical step in polymerase chain reaction or PCR. In this step, the primers bind to flanking sequences of the target DNA for amplification. If the primer annealing temperature is increased to 94°C non annealing occurs. At high temperature Non annealing occurs and the whole process is disturbed.
Last updated
About Biological Molecules
The chapter covers water's polarity and biological roles, carbohydrates, lipids, proteins and nucleic acids, including their monomers, bonds, structures and functions. It also distinguishes RNA from DNA and explains conjugated molecules such as glycoproteins, glycolipids and nucleoproteins, which combine organic molecules with a non-protein component.
Practise Biological Molecules
1,790 free Biological Molecules MCQs from Biology, each with the correct answer and an explanation. Unlimited attempts, no account needed.
Exams that ask Biology questions like this
Biology is on 6 papers prepared for on TestUstad, and all of them draw the same bank, so this question is worth knowing for every one of them.
Related questions
A common carbohydrate composed of glucose and fructose joined by a linkage between C-1 of glucose and C-2 of fructose is:
A complex aminoacid subunits is called?
A compound produced as a result of a chemical reaction of an alcohol with an acid, where a water molecule is released, is called:
A compound produced as a result of a chemical reaction of an alcohol with an acid in which water molecule is released is called_.
A dipeptide glycylalanine consists of: