The comparative rates at which the solutes move in paper chromatography, depend on:

Correct answer: B. Retention Factor

  • A. the size of paper
  • B. Retention Factor
  • C. temperature of the experiment
  • D. size of the chromatographic tank used

Explanation

The comparative rate at which the solutes move in paper chromatography mainly depends on the retention factor, which is expressed as the ratio of the distance travelled by the solute to the total distance travelled by the solvent (i.e., solvent front).

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Separation methods are selected according to differences in solubility, particle size, volatility and sublimation behaviour. Filtration and crystallisation separate insoluble or dissolved solids, sublimation isolates suitable volatile solids, distillation separates liquids by boiling point, and solvent extraction transfers a substance between immiscible solvents.

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