All Free Biology MCQs with Answers
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19844 questions · page 533 of 1985
5321. The collection of different sized fragments is:
- A. Probes
- B. RFLP
- C. PCR
- D. Gel
Explanation: The genome is treated with restriction enzymes, which resultsin a unique collection of diferent sized fragments. Therefore, restriction fragmentlength polymorphism (RFLPs) exists between individuals.
Correct answer: RFLP5322. DNA analyzer is used for:
- A. Forensic
- B. Diagnosis
- C. Evolution
- D. All of the above
Explanation: PCR amplification and analysis can be used (1) to diagnose viral infections, geneticdisorders, and cancer (2) in forensic laboratories to identify criminals; and (3) todetermine the evolutionary history of the human population
Correct answer: All of the above5323. The use of DNA analysis for convicting the criminal of rape is used in:
- A. Diagnosis
- B. Forensic
- C. Evolution
- D. All of the above
Explanation: DNA analysis is used in forensic laboratories to identify criminals.The DNA from a single sperm is enough to identify a suspected rapist
Correct answer: Forensic5324. Match Sanger's method for the generation of pieces of DNA with one of the following:
- A. Deoxyribonucleotide
- B. Ribonucleoside
- C. Dideoxyribonucleoside
- D. DNA threads
Explanation: For a generation of different-sized DNA fragments, two methods are generally used. Oneis Sanger's, method in which dideoxyribonucleoside triphosphates are used to terminateDNA synthesis at different sites
Correct answer: Dideoxyribonucleoside5325. Match Maxam Gilbert's method for the generation of pieces of DNA with one of the following:
- A. Deoxyribonucleotide
- B. Dexoyribonucleoside
- C. Dideoxyribonucleoside
- D. DNA threads
Explanation: For a generation of different-sized DNA fragments, two methods are generally used. Oneis Sanger's, method in which dideoxyribonucleoside triphosphates are used to terminateDNA synthesis at different sites The other method is known as the Maxam-Gilbert methodin which DNA threads are chemically cut into pieces of different sizes
Correct answer: DNA threads5326. Pieces of DNA are separated on:
- A. Agarol
- B. Agarose gel
- C. Agar
- D. All of the above
Explanation: Pieces of DNA are separated on agarose gel
Correct answer: Agarose gel5327. In gene sequencing the nucleotide strained with fluorescent dye are:
- A. Initiation nucleotides
- B. Middle nucleotides
- C. Terminating nucleotides
- D. None of the above
Explanation: DNA sequence is now completely automated, robotic devices mix the reagents andthen load, run and read the order of the nucleotide bases from the gel. This is facilitatedby using chain terminating nucleotides that are each labelled with a diferent coloredfluorescent dye; in this case, all four synthesis reactions can be performed in the sametube, and the products can be separated in a single lane of a gel.
Correct answer: Terminating nucleotides5328. In gene sequencing, the colour of the fluorescent bands are read by:
- A. Computer
- B. Gel
- C. Detector
- D. Laser beam
Explanation: A detector positionednear the bottom of the gel reads and records the color of the fluorescent label on each bandas it passes through a laser beam
Correct answer: Detector5329. The nucleotide sequence is stored by:
- A. Computer
- B. Gel
- C. Detector
- D. Laser beam
Explanation: A computer reads and stores the nucleotidesequence.
Correct answer: Computer5330. The DNA sequence of which of the followings have been done?
- A. Chloroplast
- B. Mitochondria
- C. Yeast
- D. All of the above
Explanation: The automation of DNA sequencing, the genomes of many organisms have been sequenced. These include plant chloroplasts and animal mitochondria, large number of bacteria, many of the yeasts, a nematode worm. Drosophila, the model plant Arabidopsis, the mouse and human. Researchers have also deduced the complete DNA sequence of a variety of human pathogens.
Correct answer: All of the above