Which of the following analytical method is based on the rotation of light radiation?

Correct answer: B. Polarimetry

  • A. Refractometry
  • B. Polarimetry
  • C. Interferometry
  • D. Mass spectrometry

Explanation

Polarimetry is a technique that measures the angle of rotation of plane-polarized light as it passes through an optically active (chiral) substance. This rotation is used to determine the concentration and purity of chiral compounds.

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Analytical chemistry identifies and measures substances using separation and instrumental methods. Chromatography is studied through stationary and mobile phases, retention and Rf values, while spectroscopy connects absorption of electromagnetic radiation with molecular structure and supports qualitative and quantitative analysis through techniques such as UV-visible and infrared spectroscopy.

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