Which of the following method is used to separate small molecules from the larger molecules in diffusing through a membrane?
Correct answer: A. Dialysis
- A. Dialysis
- B. Chromatography
- C. HPLC
- D. FPLC
Explanation
Dialysis is a separation process that uses a semipermeable membrane to separate small solute molecules from larger molecules (like proteins) in a solution based on their different rates of diffusion across the membrane.
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About Analytical Chemistry
Analytical chemistry identifies and measures substances using separation and instrumental methods. Chromatography is studied through stationary and mobile phases, retention and Rf values, while spectroscopy connects absorption of electromagnetic radiation with molecular structure and supports qualitative and quantitative analysis through techniques such as UV-visible and infrared spectroscopy.
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